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In contrast to oligo(dT) primers, these primers are usually not template specific and therefore are appropriate for DNA synthesis making use of Klenow fragments with DNA templates or for cDNA synthesis making use of reverse transcriptase with mRNA templates.
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Assist with this option on, the program will check out to find primer pairs that are separated by at the very least one intron around the corresponding genomic DNA making use of mRNA-genomic DNA alignment from NCBI.
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